Teach you tips for folic acid determination

Folic acid is one of the vitamin B complexes. It promotes the maturation of young cells in the bone marrow and improves endothelial function and prevents cardiovascular disease. People cannot synthesize themselves and need to take it from food. Humans such as the lack of folic acid can cause macrocytic anemia and leukopenia.

Folic acid is a group of compounds with similar chemical structures and similar biochemical properties. It is a combination of acridine, p-aminobenzoic acid and one or more glutamic acids. The majority of folic acid in food is in the form of decanoyl polyglutamic acid (or polyglutamic acid folate). The food folic acid is absorbed by the specific folate polyglutamate hydrolase in the small intestinal mucosa cells and hydrolyzed into decanoyl monoglutamate.

A part of the absorbed monoglutamic acid folic acid is converted to polyglutamate folic acid, which is stored in liver, red blood cells and other tissue cells, and the rest is monoglutamic acid folic acid. Forms are distributed in plasma, tissue fluid, bile and urine. The folate concentration of the liver is several hundred times that of plasma, but its monoglutamic acid folate concentration is similar to plasma.

It is reported that an adult needs about 400 micrograms of natural folic acid a day, about 600 micrograms for pregnant women and 500 micrograms for lactating women. Most of the food added is artificial folic acid, which must be converted into active natural folic acid in the human body to be utilized. Adding too much artificial folic acid is not a good thing and hinders the absorption of natural folic acid, so the American Medical Institute stipulates that the upper limit of artificial folic acid intake per day is 1000 micrograms.

Therefore, it is meaningful to detect the folate content in foods, and the determination of folic acid is complicated by the complex composition of various samples. There are many methods for determining the content of folic acid. In general, we use a microbial method to detect the content of folic acid in food.

China’s “Determination of Folic Acid in Food” standard specifies the method for determination of folic acid in food. Folic acid is an essential nutrient for the growth of Lactobacillus rhamnosus. Under certain controlled conditions, the Lactobacillus rhamnosus solution is inoculated into the culture solution containing the sample solution, and the light transmittance (or the absorbance value) is measured after a period of culture. The content of folic acid in the sample is calculated from a standard curve of folic acid content and light transmittance (or absorbance value).

The growth status of Lactobacillus rhamnosus is used to determine the folic acid content. However, we often encounter some problems during the experiment. For example, the folic acid determination medium used contains trace amounts of folic acid, which leads to high measurement results; the repeated test results have large deviations; the experimental accuracy is not high. At this time, it is very likely that there is a problem with the medium you have chosen. What we lack is a reliable folic acid assay medium.

Wei Zheng Xiang Yu|Construction of a product from HiMedia – Folic Acid Determination Medium (Cat. No. M543)

If you change the medium, is it solved?

                

                
                                    

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